Journal:

Article Title: Succession of Indigenous Pseudomonas spp. and Actinomycetes on Barley Roots Affected by the Antagonistic Strain Pseudomonas fluorescens DR54 and the Fungicide Imazalil

doi: 10.1128/AEM.67.3.1147-1153.2001

Figure Lengend Snippet: Strains tested in the Streptomyces -targeting PCR

Article Snippet: PCR conditions were specific for the species without mismatches (Table ). table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Species Strain PCR product Actinomycetes Streptomyces lavendulae DSM 40069 T + Streptomyces diastaticus DSM 40496 T + Streptomyces griseoflavus DSM 40456 T + Streptomyces albus DSM 40313 T + Streptomyces sp. DSM 40533 + Streptomyces albidoflavus DSM 40455 T + Corynebacterium glutamicum DSM 20300 + Rhodococcus sp. Our isolate + 20 filamentous actinomycete isolates a Our isolate + Arthrobacter globiformis DSM 20124 T − Micrococcus luteus DSM 20030 T − Nonactinomycetes Flavobacterium ferrugineum DSM 30193 T − Cytophaga arvensicolor DSM 3695 − Bacillus cereus ATCC 14579 T − Pseudomonas fluorescens DR54 − Open in a separate window a isolated from the same soil using water agar.

Techniques:

Journal: Microbial Cell Factories

Article Title: Comparative evaluation of the extracellular production of a polyethylene terephthalate degrading cutinase by Corynebacterium glutamicum and leaky Escherichia coli in batch and fed-batch processes

doi: 10.1186/s12934-024-02547-2

Figure Lengend Snippet: Extracellular activities determined by enzymatic hydrolysis of pNPB using E. coli and C. glutamicum cultures in stirred-tank reactors after 24 h. Different signal peptides were used for the secretion of the target protein in C. glutamicum cultures. For E. coli , induction by autoinduction and IPTG was compared. As controls, C. glutamicum was cultivated with only the vector pEKEx2 and E. coli with the vector pET26b. Each bar represents the mean ± standard deviation of three hydrolysis measurements

Article Snippet: For expression in C. glutamicum (DSM 20300, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) [ ] the gene sequence of the cutinase variant (ICCG DAQI ) [ ] used in this work was cloned into the pEKEx2 vector using E. coli DH5α MCR cells [ ].

Techniques: Plasmid Preparation, Standard Deviation